ROXY EC-MS

ROXY tm  Workstation for EC-MS

 

Simulated Oxidative Metabolism Workstation

Antec has developed a dedicated instrument for EC-MS with applications in the simulation of oxidative metabolism, protein adduct formation, protein cleavage and the investigation of Oxidative damage to DNA. The ROXY is used in conjunction with MS for detection. The system is suitable to mimic (synthesize) phase I metabolites within minutes, that otherwise are obtained by time consuming in vivo (laboratory animals) or in vitro experiments (isolated liver cells, microsomes).

Advantages

  • Fast, synthesize within µgs of material minutes vs. days or weeks using traditional methods with the µ-Prep cell
  • Zero matrix effect, no isolation steps
  • Direct identification of oxidative labile sites in drug molecule
  • Zero risk of adduct formation with cell matrix
  • High throughput screening of metabolites
 
ROXY Oxidation Unit 
 
Roxy EC/MS System

EC Oxidation

 

ROXY EC/LC-MS System

ROXY EC/LC-MS

Fig. 1: Mass traces of Tetrazepam metabolites formed in the electrochemical reaction cell of the ROXY™ system.
Traditonal Microsomal OxMet
ROXY u-Prep Cell
ROXY µ-Prep Cell

Fig. 2: Mass traces of Tetrazepam metabolites formed by the microsomal approach. Experiments were performed using pooled rat liver microsomes from male Sprague Dawley rats. Courtesy: A. Baumann, B. Schubert, H. Oberacher and U. Karst, University Münster, Münster, Germany.
To download a ROXY brochure click here
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