Michrom Columns

Michrom Column Products

Michrom manufacture a large range of Columns packed with a myriad of phases including Poly LC phases, Halo and their own Magic packing materials.
Intended for reduced bore LC, Column IDs range from 2mm to 75µm. This makes them Ideal for LC-MS applications.
 
 
Michrom Columns Dimensions and Loadings
 
Column ID (mm) Flow Rate Range* (µl/min) Optimal Flow Rate (µl/min) Capacity Range For Complex Mixtures (µg) Capacity Range of a Single Analyte (pmol)
0.075
0.125-1.25  
0.25
0.5-2  
0.05-0.125  
0.1
0.25-2.5  
0.5
1-4
0.1-0.25  
0.15
0.5-5.0  
1
2-8
0.2-0.5  
0.2
1.0-10.0  
2
4-16
0.4-1  
0.3
2.0-20.0  
5
10-40
1-2.5  
0.5
5.0-50.0  
12
25-100  
2.5-10  
1
20.0-200  
50
100-400  
10-40
2
100-1000  
200
400-1600  
40-160  
Micro Bullet  
100-1000  
500
400-1600  
40-160  
Macro Bullet  
500-5000  
2000
1600-6400  
160-640  
 
Michrom Column Packings
 
Reversed Phase -- Michrom's legendary Magic packing has highly efficient bonding and end-capping to eliminate harmful silanol group residues. An array of pore sizes, and 7 different phases (C4, C8, C18, C18AQ, C30, Phenyl, CN) assure complete separation of complex samples, or rapid screening for high, efficient throughput analysis.
 
PLRP -- Polymeric (polystyrene/divinylbenzene) media are recommended for protein separation. Its hydrophobic nature promotes RP separations without any silanol or metal reactive sites more common to silica materials. These robust particles work at elevated temperatures and pH range from 1-14 without damage to the stationary phase or background effects in the MS.
 
SCX -- PolySulfoethyl Aspartamide is a strong cation exchange material developed for peptide separations from pH range 2.7 - 4. (total range pH 2.7 - 7) Available on 3, 5, or 12 micron particles, these materials are an excellent choice for first dimension MudPIT separation.
 
Size Exclusion -- Polyhydroxyethyl Aspartamide is designed for the separation of analytes based on size and shape. Those molecules above the threshold size are not retained, therefore elution order is inversely proportional to molecular size. These materials are commonly used in the first dimension of separation to exclude unwanted analytes such as proteins from serum.
 
HILIC--Polyhydroxyethyl Aspartamide can be used in HILIC mode by using the appropriate buffers. Analytes are retained in a highly organic environment, and elute with decreasing organic content. Typically conditions for efficient peptide separations include 10 - 15 mM salt at pH 3.0
 
 
Download a complete Michrom Column Guide here